Transgenic Mice Transcribing the Human H1o Histone Gene Exhibit a Normal Phenotype

Transgenic mice carrying the apolipoprotein E3-Leiden gene exhibit hyperlipoproteinemia.

Apolipoprotein (apo) E3-Leiden, described in a large Dutch family, is associated with a dominantly inherited form of familial dysbetalipoproteinemia. To study the effect of the APOE*3-Leiden mutation in vivo, transgenic mice were generated using a genomic 27-kilobase DNA construct isolated from the APOE*3-Leiden proband. This construct carried the APOE gene, the APOC1 gene, and all known regula...

Transgenic Cystic Fibrosis Mice Exhibit

Overexpression of human loricrin in transgenic mice produces a normal phenotype.

The cornified cell envelope (CE) of terminally differentiating stratified squamous epithelial cells is a complex multiprotein assembly about 15 nm thick of which loricrin is a major component. We have produced transgenic mice bearing the human loricrin transgene in order to study the role of loricrin in CE assembly, structure, and function. By analyses of RNA and protein, we show that the human...

Histone H1o: its location in chromatin.

Histone H1o is an H5-like protein found in mammals. Its location in chromatin from animal tissues has been studied by micrococcal nuclease digestion and by quantitation. It was found that H1o occurs in the linker region of chromatin, and replaces H1 there as it does so. As much as a third of the H1 becomes replaced, at least in some tissues. The abundance of H1o was similar in bulk chromatin an...

Thermosensitive phenotype of transgenic mice overproducing human glutathione peroxidases.

Exposure of humans and other mammals to hyperthermic conditions elicits many physiological responses to stress in various tissues leading to profound injuries, which eventually result in death. It has been suggested that hyperthermia may increase oxidative stress in tissues to form reactive oxygen species harmful to cellular functions. By using transgenic mice with human antioxidant genes, we d...